Summary:
Franciele Maboni Siqueira is a distinguished researcher and academic professional with a strong background in biochemistry, bacteriology, and molecular biology. Born on April 2, 1984, in Frederico Westphalen, RS, Brazil, she has made significant contributions to the fields of infectious diseases in animals, molecular diagnostics, and animal health.
Sequential PCR Reactions:
Similar to nested-PCR, the protocol involves two sequential PCR reactions to maximize the target amplicon. This approach is used to enhance the detection of microbial DNA in low-biomass samples.
Target Regions:
The protocol targets 16S-rDNA for bacterial sequencing and the internal transcribed spacer region (ITS) for fungal sequencing. These regions are commonly used in microbiome studies to identify and characterize microbial communities.
Comparison with Original Protocol:
The study compares results obtained using the alternative protocol (P2) with the original Illumina protocol (referred to as Protocol 1 – P1). The comparison is conducted using a mock community and clinical samples with varying microbial loads.
Metagenomic Comparison:
The authors evaluate metagenomic results from both protocols and find no significant differences in the data generated by P1 and P2. This suggests that the second amplification round in the alternative protocol does not introduce biases in microbiota diversity rates.
Applicability for Low-Biomass Samples:
The findings suggest that the alternative protocol (P2) can be applied for low-biomass samples, especially when the original protocol (P1) results in spurious output, making library preparation and sequencing challenging.
Education:
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Doctorate in Biochemistry (2010 – 2013):
- University: Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil
- Title: Análise genômica e transcricional comparativa de Mycoplasma hyopneumoniae, Mycoplasma flocculare e Mycoplasma hyorhinis.
- Advisor: Arnaldo Zaha
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Master’s in Biology (2009 – 2010):
- University: UFRGS, Porto Alegre, Brazil
- Title: Organização das Unidades de Transcrição em Mycoplasma hyopneumoniae.
- Advisor: Irene Silveira Schrank
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Graduation in Medicina Veterinária (2003 – 2008):
- University: Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil
Research Focus:
Dr. Siqueira’s research primarily centers around:
- Infectious diseases of animals, with a specific focus on Mycoplasma species.
- Molecular biology applications in bacteriological diagnosis.
- Animal health and sanitary programs.
- Bacteriology, emphasizing the study of bacterial behavior and characteristics.
Professional Journey:
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Postdoctorate (2014 – 2017):
- Center: Centro de Biotecnologias, UFRGS, Brazil
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Postdoctorate (2013 – 2014):
- Center: Centro de Biotecnologias, UFRGS, Brazil
Honors & Awards:
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Menção Honrosa do Prêmio Capes de Tese 2023 (2023):
- Honorable Mention in the CAPES Thesis Award.
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Top 50 reviewers Ciência Rural (2016):
- Recognition as one of the top reviewers for Ciência Rural Journal.
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PRÊMIO COLABORADOR DESTAQUE DO LABAC (2006):
- Outstanding Collaborator Award from LABAC – Bacteriology Laboratory.
Publications Top Noted & Contributions:
Title: Biodegradation potential of oily sludge by pure and mixed bacterial cultures
Authors: VS Cerqueira, EB Hollenbach, F Maboni, MH Vainstein, FAO Camargo, …
Published in: Bioresource Technology, 102(23), 11003-11010
Year: 2011
Cited By: 343
Title: New insights on the biology of swine respiratory tract mycoplasmas from a comparative genome analysis
- Authors: FM Siqueira, CE Thompson, VG Virginio, T Gonchoroski, L Reolon, …
- Published in: BMC Genomics, 14(1), 1-17
- Year: 2013
- Cited By: 85
Title: Bioprospection and selection of bacteria isolated from environments contaminated with petrochemical residues for application in bioremediation
- Authors: VS Cerqueira, EB Hollenbach, F Maboni, FAO Camargo, MCR Peralba, …
- Published in: World Journal of Microbiology and Biotechnology, 28, 1203-1222
- Year: 2012
- Cited By: 67
Title: Genomic insights into the versatility of the plant growth-promoting bacterium Azospirillum amazonense
- Authors: FH Sant’Anna, LGP Almeida, R Cecagno, LA Reolon, FM Siqueira, …
- Published in: BMC Genomics, 12, 1-14
- Year: 2011
- Cited By: 66
Title: Antimicrobial activity of propolis extract against Staphylococcus coagulase positive and Malassezia pachydermatis of canine otitis
- Authors: RL Cardoso, F Maboni, G Machado, SH Alves, AC de Vargas
- Published in: Veterinary Microbiology, 142(3-4), 432-434
Author Metrics:
Author metrics, such as citation counts, h-index, and impact factor of journals publishing her work, would provide insights into her scholarly impact.
- Citations: 802
- Documents: 70
- h-index: 13
Research Timeline:
- 2010 – 2013: Doctorate in Biochemistry
- 2014 – 2017: Postdoctorate (1st)
- 2013 – 2014: Postdoctorate (2nd)
- 2023 – Present: Reviewer for CNPq
- 2023 – Present: Reviewer for ANII
- 2023: Honorable Mention in CAPES Thesis Award
